Norcantharidin induces apoptosis through Wnt/β-catenin signaling pathway in human ovarian cancer SKOV3 cells
10.16753/j.cnki.1008-2344.2024.04.003
- VernacularTitle:去甲斑蝥素干预Wnt/β-catenin信号通路诱导人卵巢癌SKOV3细胞凋亡
- Author:
Yueting ZHAO
1
;
Ying WANG
;
Mengting GAO
;
Xiu DONG
Author Information
1. 辽宁中医药大学研究生学院,辽宁 沈阳 110847
- Keywords:
norcantharidin;
apoptosis;
ovarian cancer SKOV3 cell;
Wnt/β-catenin signaling pathway
- From:
Journal of Shenyang Medical College
2024;26(4):346-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Norcantharidin(NCTD)on the apoptosis of human ovarian cancer SKOV3 cells and its mechanism.Methods:Human ovarian cancer SKOV3 cells were cultured in vitro and treated with 52 μmol/L NCTD.The SKOV3 cells were divided into the control group and NCTD group(52 μmol/L).The morphological changes of SKOV3 cells were observed under an inverted microscope,and the changes of nucleus were observed under a fluorescence microscope.The changes of mitochondrial membrane potential were detected by flow cytometry after drug treatment for 24 h.Western blot was used to detect the expression levels of apoptosis-related proteins Bax,Bcl-2 and Wnt/β-catenin signaling pathway-related protein β-catenin in SKOV3 cells after drug treatment for 24 h.Result:Compared with that in the control group,NCTD inhibited the proliferation of SKOV3 cells,reduced mitochondrial membrane potential,induced apoptosis of SKOV3 cells,increased the expression of Bax protein,decreased the expression of Bcl-2 protein and β-catenin.Conclusion:NCTD-induced apoptosis of ovarian cancer SKOV3 cells may be related to the regulation of Wnt/β-catenin signaling pathway.
