Application of serum N -glycan profiling diagnostic model in evaluation of liver fibrosis in patients with hepatitis C
10.3760/cma.j.cn501113-20190928-00359
- VernacularTitle:血清N-糖组诊断模型在丙型肝炎患者肝纤维化评价中的应用
- Author:
Xi CAO
1
;
Ying ZHANG
;
Yuemin NAN
;
Zongnan TAN
;
Cuiying CHEN
;
Qinghua SHANG
;
Xueen LIU
;
Hui ZHUANG
Author Information
1. 北京大学医学部基础医学院病原生物学系和感染病中心 100191
- Keywords:
Hepatitis C, chronic;
Liver cirrhosis;
Diagnostic models;
N-glycome
- From:
Chinese Journal of Hepatology
2020;28(12):1023-1029
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of serum N-glycan abundance in patients with liver fibrosis at different stages of hepatitis C, and to establish and evaluate the diagnostic model for clinical application value.Methods:Data of 169 hepatitis C virus-infected cases with liver fibrosis were enrolled. Nine kinds of serum N-glycans were detected and analyzed using DNA sequencer-assisted fluorophore-assisted capillary electrophoresis technology. A binary logistics regression method was used to establish a diagnostic model based on the changes in the relative content of N-glycans in each stage of liver fibrosis. Receiver operating characteristic curve was used to evaluate and compare the diagnostic efficacy with other liver fibrosis diagnostic models.Results:N-glycan diagnostic model (B and C) had highest AUROC= 0.776, 0.827 for distinguishing fibrosis S1~S2 to S3~S4 and S1~S3 to S4 than GlycoFibroTest (AUROC = 0.760, 0.807), GlycoCirrhoTest (AUROC = 0.722, 0.787), aspartate aminotransferase to platelet ratio index (AUROC = 0.755, 0.751), FIB-4 index (AUROC = 0.730, 0.774), and S-index (AUROC = 0.707, 0.744). However, the diagnostic efficacy of model A (AUROC = 0.752) for distinguishing fibrosis S1 with S2~S4 had lower diagnostic potency than that of the aspartate aminotransferase to platelet ratio index (AUROC = 0.807). Diagnostic efficiency was improved when the N-glycan profiling and the aspartate aminotransferase to platelet ratio index were combined to diagnose liver fibrosis in each stage, and the area under the receiver operating characteristic curve was 0.839, 0.825, and 0.837, respectively.Conclusion:The serum N-glycan profiling diagnostic model has potential clinical application value in the diagnosis of liver fibrosis in patients with hepatitis C.
