Correlation analysis of microorganisms in subgingival plaque in patients with T2DM and periodontitis
10.3969/j.issn.1001-3733.2024.06.018
- VernacularTitle:龈下菌斑中微生物与2型糖尿病患者牙周炎的相关性研究
- Author:
Minglu JIANG
1
;
Zhiwei FAN
;
Chunxia LIU
;
Xiangrui MA
;
Wenlong WANG
;
Caiyun CUI
;
Jing WANG
Author Information
1. 264003,滨州医学院附属医院口腔内科
- Keywords:
Moderate and severe periodontitis;
Type Ⅱ diabetes mellitus;
Subgingival plaque;
Microbial community;
16SrDNA sequencing
- From:
Journal of Practical Stomatology
2024;40(6):840-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the role of special microbial communities in the development of periodontitis in type 2 diabetes melli-tus(T2DM)patients.Methods:40 subjects aged 20-70 years were included and divided into 3 groups:moderate to severe periodon-titis with T2DM(SP.T2DM,n=15),moderate to severe periodontitis group(SP,n=15)and normal healthy group(N,n=10).The basic information,periodontal clinical indicators and blood sugar of the subjects were recorded.Subgingival plaque samples were col-lected,DNA samples of the plaque were extracted,and sequenced by Illumina NovaSeq6000 platform.The microbial diversity,eco-logical characteristics and functions of the plaque were analyzed by Uparse,SPSS and other softwares.Results:481 species in 22 phyla,30 classes,73 orders,129 families and 265 genera were obtained from the samples.Beta polymorphism analysis showed that the species composition of CP.T2DM group and CP group was similar.Alpha polymorphism analysis showed that the species richness and evenness in CP.T2DM group and CP group were higher than those in N group(P＜0.01).Venn diagram analysis showed that the species richness of the plaque in CP.T2DM group was the highest,followed by CP group and the lowest in N group.At the genus lev-el,Klebsiella and Bifidobacterium in CP.T2DM group were larger than those in CP group and N group(P＜0.05),and between group CP and N,P＞0.05.At the species level,the Capnocytophaga leadbetteri in CP.T2DM group was higher than that in CP group and N group(P＜0.05),between group CP and N,P＞0.05;There were some differences in the microbial community structure of subgingival plaque among the 3 groups.The species richness of subgingival flora in patients with CP and T2DM was higher than that in patients with CP and healthy people.Conclusion:The increase of Klebsiella,Bifidobacterium and Capnocytophaga leadbetter in subgingival flora of patients with moderate and severe periodontitis may be related to the development of T2DM.
