The effects of apical dentin surface morphology made by Er∶YAG laser cut on the growth of periodontal ligament cells
10.3969/j.issn.1001-3733.2024.06.009
- VernacularTitle:Er∶YAG激光根尖切除断面形貌对牙周膜细胞生长的影响
- Author:
Xiaoxiang HUANG
1
;
Zimeng HAN
;
Mengqi YU
;
Xiaoyu LUO
;
Benxiang HOU
Author Information
1. 100162 北京,首都医科大学附属北京口腔医院口腔显微诊疗中心
- Keywords:
Er∶YAG laser;
Apicoectomy;
Scanning electron microscope;
Endodontic surgery
- From:
Journal of Practical Stomatology
2024;40(6):787-792
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of the apical dentin surface morphology resected with Er∶YAG laser on the proliferation of human periodontal ligament cells(hPDLCs).Methods:66 single premolars were randomly divided into 3 groups(n=22),and the api-cal root slices were made by resection perpendicular to the root long axis 3 mm from the apex using high-speed handpiece(group A),piezosurgery(group B)and Er∶YAG laser(group C),respectively.SEM was used to observe the apical dentin surface in the aspects of debris,smear layer,dentinal tubules,cracks,ablation characteristics and the dentin surface roughness was measured.hPDLCs were clutured on the surface of the slices of the groups,CCK-8 method was used to detect cell proliferation on the samples at 24,48 and 72 h of culture,respectively.Results:The surface preparition time of group A was shorter than that of group B and C(P＜0.001).SEM observation showed that in group C,there was no residual debris or stained layer,and dentin tubule was visible on the dention sur-faces.Detritus and stained layers were observed in group A and B,and dentin tubule was not observed in group A.Cracks were observed in all the groups,but less in group C.Roughness(nm)of group C(1 487.13±295.90)was higher than that of group A and B(P＜0.001).CCK-8 assay showed that the cell proliferation(A value)of all groups increased gradually with the culture time after 24,48 and 72 h of hPDLCs seeded on the root surface.And the cell proliferation in group C was the most significant than that in group A and B(P＜0.05)at 48 and 72 h.Conclusion:The morphological performance of the apical dentin surface resected with Er∶YAG laser is more conducive to hPDLCs growth than that with the ultrasound and burs.
