Long non-coding RNA H19 regulates the osteogenic differentiation of MC3T3-E1 cells through WNT/β-catenin signaling pathway under the stimulation of AGEs
10.3969/j.issn.1001-3733.2024.06.005
- VernacularTitle:糖基化终末产物刺激下长链非编码RNA H19通过WNT/β-catenin信号通路调控MC3T3细胞骨向分化
- Author:
Chao DENG
1
;
Shiyu QIANG
;
Yifan MA
;
Xiaoxu RUAN
;
Yi SUN
;
Xuexue LI
Author Information
1. 241002 芜湖,安徽省口腔材料与应用转化工程研究中心,皖南医学院口腔医学院
- Keywords:
Advanced glycation end products;
Long non-coding RNA H19;
Mouse preosteoblasts;
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2024;40(6):765-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulatory effects of long non-coding RNA H19(LncRNA H19)on the osteogenic differentia-tion of MC3T3-E1 cells under the stimulation of advanced glycation end products(AGEs).Methods:MC3T3-E1 cells were cultured under the stimulation of 10 μg/mL AGEs.The cells were observed by alkaline phosphatase(ALP)staining after 7 d and alizarin red staining after 21 d culture respectively.Cell transfection technology was used to overexpress LncRNA H19 in the cells,RT-qPCR was used to detect the mRNA expression levels of ALP,Runx-2,OCN,SP7 before and after transfection of LncRNA H19.Western blotting was used to detect the protein expression of Runx-2 and[3-catenin in the cells.Results:Under the stimulation of AGEs,the ALP stai-ning color of MC3T3-E1 cells became lighter,the formation of calcified nodules was reduced,the mRNA expression levels of ALP,Runx-2,OCN,SP7 and LncRNA H19 were decreased(P＜0.05),and the protein expression level of Runx-2 was decreased.After transfection of LncRNA H19(lv-H19),the mRNA expression of LncRNA H19 was significantly up-regulated(P＜0.05),the mRNA expression of ALP,Runx-2,OCN and SP7 genes was increased(P＜0.05),ALP staining was deeper,and the expression of β-catenin was increased.Conclusion:Under the stimulation of AGEs,LncRNA H19 may affect the osteogenic differentiation of MC3T3-E1 cells by activating the expression of WNT/[3-catenin.
