Molecular mechanism of Cyclic RNA hsa_circ_0003221 in regulating the proliferation and apoptosis of oral squamous cell carcinoma cells by targeting miR-139-3p/IGF2BP3 axis
10.3969/j.issn.1001-3733.2024.06.004
- VernacularTitle:环状RNA hsa_circ_0003221靶向miR-139-3p/IGF2BP3轴调控口腔鳞癌细胞增殖和凋亡的分子机制
- Author:
Pu LI
1
;
Jingwen WANG
;
Yaqin ZOU
Author Information
1. 524000 湛江,广东医科大学附属医院口腔科
- Keywords:
circPTK2;
Oral squamous cell carcinoma;
Proliferation;
Apoptosis;
miR-139-3p/IGF2BP3 axis
- From:
Journal of Practical Stomatology
2024;40(6):759-764
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation of proliferation and apoptosis of oral squamous cell carcinoma(OSCC)cells by circular RNA hsa_circ_0003221(circPTK2)targeting microrNA(miR)-139-3p/IGF2BP3 axis.Methods:The mRNA expression of circPTK2,miR-139-3p and IGF2BP3 in tissues and cell lines was detected by qRT-PCR.OSCC CAL-27 cells were divided into 5 groups:sh circPTK2,sh NC,sh circPTK2+miR-139-3p inhibitor,sh circPTK2+inhibitor NC and blank control groups.Cell prolifera-tion rate,apoptosis rate,expression of related proteins and IGF2BP3 were respectively detected,and the targeting relationship between miR-139-3p and circPTK2 and IGF2BP3 was respectively verified.Results:The expression of miR-139-3p was decreased in OSCC tissues and the OSCC cell lines,and the mRNA expression of circPTK2 and IGF2BP3 was increased(P＜0.05).Silencing circPTK2 inhibited cell proliferation,decreased mRNA and protein expression of circPTK2 and IGF2BP3,and promoted cell apoptosis and miR-139-3p expression(P＜0.05).miR-139-3p inhibitor reversed the inhibitory effect of silencing circPTK2 on the malignant behavior of CAL-27 cells(P＜0.05).miR-139-3p has a targeting relationship with circPTK2 and IGF2BP3,respectively.Conclusion:Silencing circPTK2 can regulate OSCC cell proliferation and apoptosis through miR-139-3p/IGF2BP3 axis.
