Relationship between sugar metabolism and acid production and cariogenicity of Prevotella denticola
10.3969/j.issn.1001-3733.2024.06.003
- VernacularTitle:栖牙普雷沃菌的代糖产酸与致龋关系研究
- Author:
Yuan SI
1
;
Yanfei SUN
;
Xuejiao SONG
;
Junli WAN
;
Min LI
;
Fang YANG
Author Information
1. 266023,青岛大学口腔医学院
- Keywords:
Dental caries;
Prevotella denticola;
Sugar metabolism;
Acid production;
Demineralization
- From:
Journal of Practical Stomatology
2024;40(6):753-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential relationship between sugar metabolism,acid production and cariogenicity of Prevotella denticola.Methods:Morphological features of Prevotella denticola were observed and respectively cultured under incubation conditions with and without sugar and at different pH values.The growth characteristics of Prevotella denticola were detected by UV-Vis spectro-photometer and pH meter,the organic acid content in the culture supernatants of the cultures was detected by HPLC.Dentin slices were divided into control group,phosphoric acid group and the Prevotella denticola group and cultured in the corresponding mediu for 1 and 2 weeks respectively,the degree of demineralization of the samples was examined SEM and VHM.Results:Prevotella denticola fermen-ted sucrose and glucose,produced acids with its final pH values as low as 4.7,Succinic acid and acetic acid were its main metabolites.Prevotella denticola was moderately acid-tolerant.Furthermore,Prevotella denticola was able to cause dentin demineralization,and the Vickers hardness value of dentin samples in the Prevotella denticola group was significantly decreased compared with the control group(P＜0.05).Conclusion:The cariogenic capacity of Prevotella denticola may be related to its sugar metabolism and acid production.
