Single-cell level analysis of the immune microenvironment characteristics of dental follicle tissues from the human third molars
10.3969/j.issn.1001-3733.2024.06.002
- VernacularTitle:单细胞水平解析人第三磨牙牙囊组织免疫微环境特征
- Author:
Jianing LIU
1
;
Xiaohui ZHANG
;
Yuan CAO
;
Lu LIU
;
Xiao LEI
;
Jiongyi TIAN
;
Junxi HE
;
Fang JIN
;
Bingdong SUI
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学第三附属医院组织工程中心
- Keywords:
Single-cell RNA sequencing;
Dental follicle;
Immunity;
Microenviron-ment
- From:
Journal of Practical Stomatology
2024;40(6):747-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the immune microenvironment characteristics of human dental follicle tissues from the third molars and to explore the mutual communication and the effects of innate immune cells and adaptive immune cells within the dental follicle.Methods:Sequencing data(GSA-Human:HRA008022)in the GSA database were analyzed.Bioinformatics tools were employed for gene identification and GO enrichment analysis was performed to define the biological function of innate and adaptive immune cells.CellChat analysis was used for explaining intercellular communication among immune cell populations.Results:Using t-SNE dimen-sionality reduction analysis for immune cell populations,innate immune cell populations were obtained,including innate lymphoid cells,dendritic cells,mast cells and macrophages,and adaptive immune cell populations including T cells and B cells.Pearson corre-lation analysis showed that innate immune cells,specifically innate lymphoid cells and macrophages,had a strong correlation with adap-tive immune cell populations.GO enrichment analysis revealed mutual coordination among innate immune cell populations and regulato-ry effects on adaptive immune cell populations.Further CellChat analysis indicated biological signal transmission between innate and a-daptive immune cell populations,with CLEC,MIF,ADGRE5,COLLAGEN and MIF signaling pathways is the most significant.Con-clusion:Dental follicle tissues are rich in immune cells and innate immune cell populations interact with adaptive immune cells to regulate immune responses and participate in maintaining the homeostasis of dental follicle.
