Construction of apoptosis-stimulating of p53 protein 2 gene knockout mice and its effect on diethylnitrosamine-induced liver cancer
10.3760/cma.j.cn501113-20190728-00274
- VernacularTitle:p53凋亡刺激蛋白2基因敲除小鼠的构建及其对二乙基亚硝胺诱导肝癌的影响
- Author:
Xiaoni LIU
1
;
Buxin KOU
;
Mengyin CHAI
;
Dexi CHEN
Author Information
1. 首都医科大学附属北京佑安医院 北京市肝病研究所 100069
- Keywords:
Hepatocellular carcinoma;
Apoptosis stimulating protein of p53 2;
Gene knockout;
CRISPR/Cas9 system;
Diethylnitrosamine
- From:
Chinese Journal of Hepatology
2020;28(9):784-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct apoptosis-stimulating of p53 protein 2 (ASPP2) gene knockout mice using diethylnitrosamine (DEN)-induced liver cancer model to study the biological functions of ASPP2.Methods:The sgRNA oligonucleotides were constructed, and ASPP2 knockout mice were prepared with the CRISPR/Cas9 system. PCR and sequencing methods were used to identify the genotypes of F0 and F1 generations and their progeny. DEN was used to induce ASPP2+/- mice to establish liver cancer model.Results:PCR and sequencing results showed that ASPP2 gene was successfully knocked out in F0 generation mice. The genotype of F1 generation mice was accorded with ASPP2+/- and had obtained stable heredity. The success rate of DEN-induced liver cancer model (7/8 and 3 / 8) of ASPP2 + /-mice obtained by self-hybridization of F1 generation was significantly higher than that of wild-type mice.Conclusion:ASPP2 knockout mice were successfully constructed based on the CRISPR/Cas9 system. The success rate of DEN-induced liver cancer model of ASPP2 knockout mice was significantly higher than that of the wild-type mice.
