An experimental study on TRIM21 promoting M1 polarization of macrophages to aggravate chronic apical periodontitis
10.3969/j.issn.1001-3733.2024.05.009
- VernacularTitle:TRIM21促进巨噬细胞M1极化加剧慢性根尖周炎进展的实验研究
- Author:
Jingying ZHANG
1
;
Xiaochuan LIU
;
Xiaoqin XU
;
Peiqi LIANG
;
Jinsi WANG
;
Hong ZHU
;
Yueying WANG
;
Zhuguo WU
Author Information
1. 523710,广东医科大学附属东莞第一医院口腔科
- Keywords:
TRIM21 protein;
Chronic apical periodontitis;
Raw264.7 cells;
Bone marrow-derived macrophages;
Polarization
- From:
Journal of Practical Stomatology
2024;40(5):652-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of tripartite motif-containing protein 21(TRIM21)in chronic apical periodontitis(CAP)and its potential mechanism.Methods:Human CAP tissue and normal periodontal tissue were collected.The expression of inflamma-tory factors(IL-1 β,IL-6,TNF-a,TGF-β1),osteoclast related genes(TRAP,RANKL,CTSK),macrophage polarization related genes(CD86,iNOS,CD206,Arg1)and TRIM21 were detected by RT-qPCR.TRIM21 protein was detected by immunohistochemical staining,and the osteoclasts was detected by tartrate resistant acid phosphatase(TRAP)staining.The inflammation cell model was es-tablished by stimulating Raw264.7 cells with lipoteichoic acid(LTA)or lipopolysaccharide(LPS),and the expression of the above factors was detected.The bone marrow-derived macrophages(BMDMs)extracted from wild-type and TRIM21-/-mice stimulated by LPS were used to verify the expression of the above factors by RT-qPCR,the osteoclasts were detected by TRAP staining,and the po-larization of macrophages was detected by immunofluorescence staining.Results:In CAP tissue the expression of inflammatory factors,osteoclast related genes,CD86,iNOS and TRIM21 increased,while CD206 and Arg1 decreased,and osteoclasts were more than that in normal tissue.The stimulation of LTA/LPS promoted the proliferation of Raw264.7 cells,and the expression of these factors in cells was consistent with that in tissues.After LPS stimulation,BMDMs of TRIM21-/-mice had lighter inflammation,lower expression of os-teoclast specific genes,fewer osteoclasts and lower M1 polarization than those of wild type mice.Conclusion:TRIM21 might promote the progress of CAP by promoting M1 polarization of macrophages.
