The mechanism of metformin-mediated osteogenic differentiation of rat jaw BMSCs under high-glucose and high-fat environment
10.3969/j.issn.1001-3733.2024.05.008
- VernacularTitle:二甲双胍介导高糖高脂环境下大鼠颌骨BMSCs成骨分化的机制研究
- Author:
Dongjie QI
1
;
Xinlei ZHOU
;
Buyun JI
;
Jiating LIN
Author Information
1. 241002 芜湖,皖南医学院口腔医学院
- Keywords:
Metformin;
Runx2;
OCN;
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2024;40(5):647-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of metformin on osteogenic differentiation of rat jaw bone marrow mesenchymal cells(BMSCs)under high sugar and high fat environment.Methods:BMSCs of male SD rats were isolated and cultured in vitro and divided into 4 groups:Control group(C group),high sugar and high fat group(H group),control cells and H group cells were respectively stimulated with metformin(CM group and HM group).EDU staining was used to detect the cell proliferation;after osteogenesis and adi-pogenesis induction of BMSCs,alkaline phosphatase staining,alizarin red(O)staining and oil red O staining were respectively used to detect the osteogenic and adipogenic differentiation of the cells.The protein expression of Runx2 and OCN was detected by Western blot and immunofluorescence staining respectively.Results:Compared with the H group,in the HM group the cell proliferation ability was enhanced,the expression levels of Runx2 and OCN were increased(P＜0.05),the osteogenic differentiation ability was significantly in-creased,and the adipogenic differentiation ability was significantly decreased.Conclusion:In vitro,metformin promotes the prolifera-tion and osteogenic differentiation of rat jaw BMSCs cultured with a high sugar and high fat environment by increase of the expression of Runx2 and OCN.
