Preparation and characterization of an injectable enamel matrix protein hydrogel

VernacularTitle:可注射釉基质蛋白水凝胶制备及其性能研究
Author: Chunbao WANG ¹ ; Ziyu ZENG ; Yaqi QUAN ; Zhongshan WANG
Author Information

1. 336000,宜春学院化学与生物工程学院
Keywords: Poloxamer; Thermosensitive injectable hydrogel; Enamel matrix protein; Release performance
From: Journal of Practical Stomatology 2024;40(5):614-618
CountryChina
Language:Chinese
Abstract: Objective:To prepare an enamel matrix protein(EMP)loaded poloxamer thermosensitive injectable hydrogel,and to study its characteristics.Methods:EMP was extracted from a 6-month old porcine and identified by SDS-PAGE electrophoresis.EMP at 2 mg/mL was loaded into gels poloxamer with P407∶P188 of 23∶8 and 19∶1 respectively.The gelling temperature of blank gel and EMP gel was detected by an inverted tube method.The release performance of selected EMP-loaded gel was detected by an Ultra-micro spectrophotometer.Results:A hydrogel(code number:FP1)with formula of 2 mg/mL EMP and 23∶8 of P407∶P188 possessed gel-ling temperature of 33 ℃,and the release behavior of FP1 conformed to the Higuchi equation.Conclusion:A poloxamer thermosensitive injectable hydrogel was successfully fabricated,this formulation possesses adaptive phase inversion temperature and controlled release ef-ficiency.