Non-target metabolomic analysis of buccal pouch squamous cell carcinoma in Chinese hamsters
10.3969/j.issn.1001-3733.2024.05.002
- VernacularTitle:中国地鼠口腔颊囊鳞癌组织的非靶代谢组学分析
- Author:
Ruihu ZHANG
1
;
Yuan WANG
;
Guoqiang XU
;
Jiping GAO
;
Guohua SONG
;
Zhaoyang CHEN
;
Rui-Jing XUAN
Author Information
1. 030001 太原,实验动物与人类疾病动物模型山西省重点实验室,山西医科大学实验动物中心
- Keywords:
Chinese hamsters;
Oral squamous cell carcinoma;
Non-target metabolome
- From:
Journal of Practical Stomatology
2024;40(5):608-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the metabolic basis and related molecular mechanism of oral squamous cell carcinoma(OSCC)path-ogenesis.Methods:20 Chinese hamsters were divided into 2 groups(n=10).OSCC models were induced by dimethylbenzanthracene(DMBA)in 10 of the animals and the other 10 were used as the controls.LC-MS chromatography-mass spectrometry was used to iden-tify the metabolites in the buccal pouch,and multidimensional statistical analysis of the metabolites was performed with the orthogonal partial least squares discriminant analysis model.Variable Importance for the Projection(VIP)＞1 and P＜0.05 were used as the criteria to screen the differential metabolites between the 2 groups.KEGG pathway annotation and enrichment analysis for the metabolites were performed to screen the significantly differential pathways.Results:The hamster cheek pouches painted with 0.5％DMBA for 18 weeks were diffused with leukoplakia and loaded obvious papillary protrusions,which were diagnosed as OSCC by pathological examination.Lipids and lipid-like molecules were the main differential metabolites.Reprogramming of unsaturated fatty acid biosynthesis,cholesterol accumulation,enhanced catabolism of tryptophan,up-regulation of aspartate,increased synthesis of pyrimidine and purine,etc.were important metabolic features in the occurrence and development of OSCC.Conclusion:Molecular intervention targeting the related met-abolic pathways is expected to inhibit OSCC pathogenesis and progression.
