Correlational analysis of alternation of clinical features of intrahepatic lymphocyte subsets with HBV virology and liver fibrosis in HBV-Tg composite CCl 4 mice model
10.3760/cma.j.cn501113-20190723-00265
- VernacularTitle:HBV-Tg复合四氯化碳模型小鼠肝内淋巴细胞亚群变化特点及其与HBV病毒学和肝纤维化的相关性分析
- Author:
Xin SUN
1
;
Kai HUANG
;
Man ZHANG
;
Hongliang LIU
;
Zhimin ZHAO
;
Yanyan TAO
;
Yuan PENG
;
Chenghai LIU
Author Information
1. 上海中医药大学附属曙光医院肝病研究所 201203
- Keywords:
Hepatitis B virus;
Liver fibrosis;
Lymphocytes;
Transgenic animal;
Carbon tetrachloride
- From:
Chinese Journal of Hepatology
2020;28(7):580-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the alteration of clinical features of intrahepatic lymphocyte subsets in C57BL/6N-TG (1.28HBV)/Vst hepatitis B virus (HBV) transgenic mice composite carbon tetrachloride (CCl 4) with intraperitoneal injection under the background of hepatitis B to induce liver fibrosis mice model, and analyze their correlation with serum HBV DNA and liver tissue hydroxyproline (Hyp) content. Methods:HBV-Tg mice were intraperitoneally injected with 10% CCl 4 to induce the rapid formation of hepatic fibrosis. Serum HBV DNA, HBsAg, HBeAg levels and liver tissue HBsAg expressional conditions were used to evaluate the virological characteristics of mice model. The degree of hepatic inflammation and fibrosis in mice were observed by HE, Sirius Red staining and liver tissue hydroxyproline (Hyp) content. Intrahepatic T lymphocyte, B lymphocyte, CD4+T lymphocyte, CD8+T lymphocyte, natural killer (NK) cell and natural killer T (NKT) cells distribution were observed by flow cytometry. One-way analysis of variance was used for intergroup data comparison, and LSD was used for pairwise comparison. Pearson’s correlation analysis was used to analyze the correlation between the above lymphocyte subsets and serum HBV DNA and liver tissue Hyp content. Results:Serum HBsAg, HBeAg and liver tissue HBsAg had equal positive expression in the HBV-Tg composite CCl 4 mice model group, and the serum HBV DNA load was > 1 × 10 6 IU / ml. Compared with the wild-type control group, liver tissue Hyp content of the composite model group was significantly higher [(196.39 ± 38.14) μg /g and (347.67 ± 59.53) μ g/g, P < 0.01). The degree of inflammation and fibrosis in liver tissues was aggravated, and the proportion of all intrahepatic CD4+T, NK and NKT cells was significantly reduced ( P < 0.01), while the proportion of CD8+T lymphocytes (30.58% ± 2.89% vs. 46.50% ± 2.24%, P < 0.01) and B lymphocytes (28.82% ± 2.24% vs. 37.10% ± 8.59%, P < 0.05) was significantly increased. Serum HBV DNA level was positively correlated with the proportion of intrahepatic T lymphocytes ( r = 0.413, P < 0.05), and negatively correlated with the proportion of NK cells ( r = -0.419, P < 0.05). Liver tissue Hyp content was negatively correlated with the proportion of all CD4+T lymphocytes ( r = -0.871), NK cells ( r = -0.716), and NKT cells ( r = -0.876) (all P < 0.01), and positively correlated with the proportion of all CD8 + T lymphocytes ( r = 0.852), and B lymphocytes ( r = 0.593) (all P < 0.01). Conclusion:HBV-Tg composite CCl4 mice model can induce positive HBV virological indicators, liver inflammation and fibrosis in mice model of hepatitis B coexisting with fibrosis. This model has the features of immune disorder of liver lymphocyte similar to human disease, and the immune disorder of intrahepatic lymphocytes is correlated with HBV viral load and liver fibrosis degree.
