Melatonin regulates osteogenic differentiation of hDPSCs through Wnt/β-catenin signaling pathway
10.3969/j.issn.1001-3733.2024.04.006
- VernacularTitle:褪黑素经Wnt/β-catenin信号通路调控hDPSCs的成骨分化
- Author:
Wanxin LI
1
;
Simei LI
;
Hongwei PAN
;
Jia WANG
;
Lihua YIN
Author Information
1. 730000,兰州大学口腔医学院(口腔医院)口腔种植科
- Keywords:
Melatonin;
hDPSCs;
Proliferation;
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2024;40(4):488-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulatory effects and mechanism of melatonin on the proliferation and osteogenic differentiation of human dental pulp stem cells(hDPSCs).Methods:hDPSCs were cultured in vitro.The cells in the blank control groups were rou-tinely cultured,while the cells in the experimental groups were cultured with osteogenic induction medium(OIM)and OIM with me-latonin at 5,1,10,25,50,100,500 and 1 000 μmol/L respectively.The effect of melatonin on the proliferation of hDPSCs was ob-served by CCK-8 assay.Alkaline phosphatase(ALP)test,RT-qPCR and Western blot were used to analyze the expression of osteo-genic related genes and proteins.The expression level of the proteins related to classical Wnt pathway was detected.Results:Melato-nin at 100 and 250 μmol/L promoted the proliferation of hDPSCs,at 100 μmol/L increased the expression levels of osteogenesis-related mRNA and proteins in hDPSCs,up-regulated β-catenin and down-regulated GSK-3β expression.Conclusion:Melatonin at specific concentrations can promote the proliferation of hDPSCs and has the potential to regulate Wnt/β-catenin signal pathway to pro-mote osteogenic differentiation of hDPSCs.
