Osteogenic activity of magnesium loaded hydrogel core-shell microspheres by microfluidic chip in vitro
10.3969/j.issn.1001-3733.2024.03.008
- VernacularTitle:微流控芯片制备载镁水凝胶核-壳微球的体外成骨活性研究
- Author:
Long LI
1
;
Hai LIANG
;
Bin LIU
;
Jian WU
;
Jie ZHU
;
Zhengjie LIN
Author Information
1. 518067,深圳市前海蛇口自贸区医院口腔科
- Keywords:
Microflluidic chip;
Hydrogels;
Osteogenic activity;
Bone regeneration;
Magnesium ions
- From:
Journal of Practical Stomatology
2024;40(3):351-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the osteogenic activity of in vitro cultured preosteoblasts treated by magnesium loaded hydrogel core-shell microspheres.Methods:A microfluidics chip device was designed and made to fabricate magnesium loaded hydrogel core-shell microspheres to precisely control the stable release of magnesium ions in vitro,the mechanism of controllable magnesium ion release in the promotion of osteogenic activity of MC3T3-E1 preosteoblasts was systematically investigated.Results:The magnesium loaded hydro-gel microspheres(PLGA/MgO-alginate microspheres)prepared by the microfluidics chip exhibited to be monodisperse with special core-shell structure and could control the stable release of magnesium ions at a concentration of 50 ppm in vitro.The in vitro study showed that it significantly enhanced the activity and proliferation ability of MC3T3-E1 preosteoblasts,promoted their osteogenic differ-entiation and mineralization ability,and increased ALP activity.Conclusion:Magnesium loaded hydrogel core-shell microspheres can promote the osteogenic activity of MC3T3-E1 osteoblasts in vitro.
