Integrated analysis of DNA methylation and gene expression profiles of hepatocellular carcinoma to construct miR-1180-3p relevant ceRNA regulatory network
10.3760/cma.j.cn501113-20200103-00003
- VernacularTitle:整合分析肝细胞癌DNA甲基化谱和基因表达谱构建miR-1180-3p相关ceRNA调控网络
- Author:
Zihan ZHOU
1
;
Xianguo ZHOU
;
Zewen ZHOU
;
Moqin QIU
;
Yanji JIANG
;
Qiuling LIN
;
Yingchun LIU
;
Qiuping WEN
;
Rongrui HUO
;
Xiumei LIANG
;
Hongping YU
Author Information
1. 广西医科大学附属肿瘤医院，南宁 530021；广西医科大学公共卫生学院，南宁 530021
- Keywords:
Hepatocelluar carcinoma;
Prognosis;
DNA methylation
- From:
Chinese Journal of Hepatology
2020;28(6):481-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study analyzes the expression level of miR-1180-3p and constructs the regulatory network of relevant ceRNA by integrating the DNA methylation and gene expression profile of hepatocellular carcinoma from the Cancer Genome Atlas (TCGA).Methods:Firstly, the expression level of miR-1180-3p in hepatocellular carcinoma and adjacent tissues was analyzed by TCGA database, and the differential expression of lncrna and mRNA was screened. Secondly, the LncBase database and the TargetScan database were used to predict the relationship between miR-1180-3p and lncRNA and mRNA, and the DNA methylation-mediated lncRNA was screened by the DNA methylation profile of lncRNA. Finally, Cytoscape software was used to construct miR-1180-3p relevant ceRNA network, and WebGestalt website was used to perform GO and KEGG analysis of related mRNA in ceRNA.Results:Compared with patients with low expression of miR-1180-3p (mean overall survival duration, 5.69 ± 0.35 years), patients with high expression of miR-1180-3p had shorter overall survival time (mean overall survival duration, 3.99 ± 0.47 years), indicating that the high expression of miR-1180-3p was hepatocellular carcinoma risk factor affecting the prognosis ( HR = 1.28, 95% CI = 1.1 ~ 1.5, P < 0.01). A miR-1180-3p related ceRNA regulatory network was constructed in this study, which contained 2 lncRNAs (F11-AS1 and LINC01511) and 37 mRNAs. Conclusion:This study has successfully constructed miR-1180-3p relevant ceRNA regulatory network, and DNA methylation-mediated F11-AS1 and F11-AS1/miR-1180-3p/C11of54 ceRNA regulatory axis has played an important role in the occurrence and development of hepatocellular carcinoma.
