Study of Ctsk knockout regulating type H blood vessel formation and tooth extraction socket healing in mice
10.3969/j.issn.1001-3733.2024.03.005
- VernacularTitle:Ctsk基因敲除调控H型血管参与小鼠牙槽窝愈合的研究
- Author:
Wuyang ZHANG
1
;
Dengke LI
;
Yiming WANG
;
Yuan LI
;
Yuzhe CHEN
;
Xueni ZHENG
;
Hongzhi ZHOU
;
Kaijin HU
;
Yang XUE
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学第三附属医院口腔颌面外科
- Keywords:
Cathepsin K;
Healing of tooth extraction;
Type H blood vessel;
Bone regeneration
- From:
Journal of Practical Stomatology
2024;40(3):330-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of cathepsin K(CTSK)on the healing process of tooth extraction socket and type H blood vessel angiogenesis in mice.Methods:Ctsk knockout(Ctsk-/-)mice were generated by CRISPR/Cas9 technology,and genotype sequen-cing,general observation,Micro-CT and immunohistochemistry were performed to confirm successful knockout of Ctsk.Then 8 week-old WT and Ctsk-/-mice were used to establish the tooth extraction modle by extracting the left maxillary first molars,and the mice were sac-rificed at the day 7,10,14,21,28 and 35 respectively(n=3)after tooth extraction.Then samples were subjected to stereo microscope and Micro-CT examination.Immunofluorescence staining was used to study the effect of Ctsk knockout on type H blood vessel angiogene-sis.Results:Ctsk knockout did not affect the soft tissue healing of tooth extraction socket,but significantly promoted the bone healing process,and Ctsk deficency significantly enhanced type H blood vessel angiogenesis in the tooth extraction socket.Conclusion:Ctsk knockout can enhance type H vessel angiogenesis,and promote bone healing process of tooth extraction socket in mice.
