The effects of SHED-EXO on subchondral bone homeostasis during rat TMJ OA
10.3969/j.issn.1001-3733.2024.03.003
- VernacularTitle:脱落乳牙牙髓干细胞来源外泌体对大鼠TMJ OA软骨下骨稳态的影响
- Author:
Yuchen DUAN
1
;
Rui HE
;
Xiaohua CHEN
;
Feng HE
;
Fan WU
;
Ying ZHAN
;
Hui MIAO
;
Shibin YU
;
Jianliang PANG
Author Information
1. 100142 北京,安徽医科大学第五临床学院,空军特色医学中心口腔科
- Keywords:
SHED-EXO;
Bone homeostasis;
Exosomes;
Osteoarthritis;
Tem-poromandibular joint
- From:
Journal of Practical Stomatology
2024;40(3):315-322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of intra-articular injection of exosomes derived from dental pulp stem cells from hu-man exfoliated deciduous teeth(SHED-EXO)on subchondral bone homeostasis in rat temporomandibular joint osteoarthritis(TMJ OA)process.Methods:36 male SD rats were randomly divided into 3 groups(n=12):control(CON),sodium iodoacetate(MIA)-induced TMJ OA(MIA),and SHED-EXO injection into TMJ OA(SHED-EXO)groups.At 2 and 6 weeks post-treatment,Micro-CT,Double labeling,TRAP staining,and immunohistochemical staining were employed to detect osteoclasts and osteoblasts in the subchondral bone.Additionally,the mRNA expression levels of ADAMTs5,IL-1β,OCN and OPG/RANKL were analyzed by qRT-PCR.Results:The MIA group exhibited significant bone loss and an enlarged bone marrow cavity.In comparison with the CON group,BV/TV and Tb.Th were lower(P＜0.001),while BS/BV,Tb.Sp,and Tb.N were higher(P＜0.01).Additionally,the bone formation rate within 5 days was low-er than that of the control group(P＜0.001).When compared to the MIA group,the SHED-EXO group showed a significant increase in bone morphology and bone mass.BV/TV and Tb.Th were increased(P＜0.01),while BS/BV,Tb.Sp and Tb.N were decreased(P＜0.05).The bone formation rate was higher(P＜0.01).Compared with both the control and treatment groups,the MIA group exhibited a significant increase in the number of osteoclasts in the subchondral bone(P＜0.01),along with a notable decrease in H-type blood vessels and OCN-positive areas(P＜0.01).Conclusion:Intra-articular injection of SHED-EXO can reg-ulate condylar subchondral bone homeostasis in TMJ OA of rats by promoting osteogenesis and inhibiting osteoclasts.
