miR-148a-3p inhibits the proliferation,invasion and metastasis of human salivary adenoid cystic carcino-ma cells by targeting EGFR
10.3969/j.issn.1001-3733.2024.03.002
- VernacularTitle:miR-148a-3p调控EGFR抑制涎腺腺样囊性癌细胞的增殖、侵袭和转移
- Author:
Wanpeng GAO
1
;
Qi ZHAO
;
Qi XI
;
Jiale WANG
;
Jiafei GUO
;
Zihui YANG
;
Jun WANG
;
Jianhua WEI
;
Xinjie YANG
Author Information
1. 154000,佳木斯大学附属口腔医学院·附属口腔医院黑龙江省口腔生物医学材料及临床应用重点实验室
- Keywords:
Adenoid cystic carcinoma of sali-vary gland;
miR-148a-3p;
EG-FR;
Proliferation;
Invasion;
Mi-gration
- From:
Journal of Practical Stomatology
2024;40(3):309-314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects and the related molecular mechanisms of miR-148a-3p on the proliferation,invasion and migration of salivary adenoid cystic carcinoma SACC-LM cells.Methods:miR-148a-3p mimics and inhibitors,siRNA targeting EG-FR and their corresponding controls were transfected into SACC-LM cells.Bioinformatics was used to predict the potential target genes of miR-148a-3p.EGFR and miR-148a-3p mRNA expression levels were examined by qRT-PCR and the protein levels of EG-FR were detected by Western blotting.CCK-8,scratch,and Transwell assays were used to study the proliferation,migration,and invasion of SACC-LM cells,respectively.The direct targeting relationship between miR-148a-3p and EGFR was examined by using the double luciferase reporter gene assay.Statistical analysis of the data was performed by SPSS 22.0 software.Results:Overexpres-sion or inhibition of miR-148a-3p significantly inhibited or promoted the proliferation,invasion and metastasis of SACC-LM cells re-spectively(P＜0.05).Bioinformatics and double luciferase assay showed that miR-148a-3p directly targeted and regulated the expres-sion of EGFR(P＜0.001).Downregulation of EGFR inhibited the proliferation,migration and invasion of SACC-LM cells(P＜0.05)and partially reversed the promoting effect of miR-148a-3p inhibition(P＜0.05).Conclusion:The downregulation of miR-148a-3p leads to the abnormally high expression of its target gene EGFR,and promotes the proliferation,invasion,and migration of salivary adenoid cystic carcinoma cells.
