Effect of knockdown of O-Glcnac transferase on hepatocyte fat synthesis
10.3760/cma.j.issn.1007-3418.2020.02.009
- VernacularTitle:敲减OGT对肝细胞脂肪合成的作用研究
- Author:
Xiangxia MIAO
1
;
Lanjing MA
;
Jiayi CAO
;
Chenchen WANG
;
Yongzhan NIE
;
Kaige LIU
Author Information
1. 西安医学院第一附属医院消化内科 710077
- Keywords:
Fatty liver,non-alcholic;
Fat synthase;
O-GlcNAc glycosylation;
O-Glcnac transferase
- From:
Chinese Journal of Hepatology
2020;28(2):147-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of knockdown of O-GlcNAc transferase (OGT) on hepatocyte fat synthesis.Methods Liver cell line L02 were used to established the model of hepatic steatosis.The levels of OGT and O-GlcNAc protein were detected by Western blot.The OGT knockdown cell line of L02 cells was established,and its lipid formation ability was detected after induction of oleic acid (OA).Real-time quantitative PCR (qRT-PCR) and Western blot were used to detect mRNA and protein expression of enzymes related to fat synthesis.An independent sample t test was used.Results Western blot showed that the expression of OGT and O-GlcNAc was increased in L02 cells after adipogenesis (P ＜ 0.05).After shOGT lentivirus infects L02 cells,OGT mRNA levels were down-regulated (P ＜ 0.01).Oil red O staining showed that the lipid in L02 shOGT cells decreased,qRT-PCR showed that the mRNA expressions of fat synthase (ACC1),(FASN) and (SCDl) were decreased,the difference was statistically significant (P ＜ 0.05),protein Expression is consistent with mRNA expression.Conclusion Knockdown of OGT can inhibit hepatocyte fat synthesis by reducing O-GlcNAc levels.
