The Effect of Green Tea Extract on Cisplatin in Cervical Cancer Cell Lines.
- Author:
Tae Gyu AHN
1
;
Dong Won KIM
;
Byoung Rai LEE
;
Sei Jun HAN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Gwangju, Korea. sjhan@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Cisplatin;
Green tea polyphenol;
HeLa cell lines;
SiHa cell lines
- MeSH:
Anti-Bacterial Agents;
Cell Culture Techniques;
Cell Line*;
Cell Survival;
Cisplatin*;
Guanosine Triphosphate;
HeLa Cells;
Humans;
Penicillins;
Streptomycin;
Tea*;
Uterine Cervical Neoplasms*;
Water
- From:Korean Journal of Obstetrics and Gynecology
2006;49(3):592-598
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Green tea polyphenol (GTP) has been shown to have anti-tumor properties in a wide variety of experimental systems. In this study, we evaluated the effects of GTP on the cytotoxic effects of cisplatin in cultured HeLa and SiHa cells. METHODS: The cell lines from Korean Cell Culture Bank were cultured in a RPMI-1640 medium supplemented with a 10% fetal bovine serum, antibiotics streptomycin and penicillin. GTP was extracted from tea leaves (Camellia scinensis) by water extraction and organic solvent fractionation. Cells were seeded at 1 x 10(4) cells/well in RPMI1640 media in triplicate wells on a Nunc Labware 96 well flat bottom microculture plate, with and without GTP (100 microgram/mL) and at different concentrations of cisplatin (0-1000 microgram/mL). After incubating the plates at 37 degrees C in 5% CO2 for 2 days, cell viability was determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; thiazolyl blue] assay. RESULTS: The viability of the HeLa cells was decreased to 14% at a 600 microgram/mL concentration of cisplatin, and to 16% at 600 microgram/mL in the SiHa cells as measured by the MTT assay. However, in the HeLa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 68% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin. And in the SiHa cell, co-cultured with GTP (100 microgram/mL), the cell viability decreased to 48% at 200 microgram/mL of cisplatin and to 17% at 400 microgram/mL of cisplatin. CONCLUSION: This study showed that cisplatin with GTP seems to have a potentiating effect on Cisplatin cytotoxicity than cisplatin alone.
