Effect of microRNA-27a-3p on proliferation, apoptosis and cell cycle of hepatoma cells
10.3760/cma.j.issn.1007-3418.2019.03.006
- VernacularTitle:微小RNA-27a-3p对肝癌细胞增殖、凋亡及细胞周期的影响
- Author:
Zhifang YANG
1
;
Ying YANG
;
Ruili ZHANG
;
Chunli JIA
;
Zhipeng LI
;
Wenran WANG
;
Hua ZHANG
;
Shaoshan LI
;
Yongxing BAO
Author Information
1. 新疆医科大学第一附属医院肿瘤中心
- Keywords:
Carcinoma,hepatocellular;
Proliferation;
Apoptosis;
Cell cycle;
MiRNA-27a-3p
- From:
Chinese Journal of Hepatology
2019;27(3):198-203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect ofmiR-27a-3p on proliferation,apoptosis and cell cycle of hepatoma cells.Methods A quantitative real-time polymerase chain reaction (qPCR) was used to detect differential expression of miR-27a-3p in normal hepatic epithelial cells (L02) and hepatoma cells (HepG2 and PLC).Cell experiment was divided into four groups:HepG2 overexpression cells,Mi-27a-3p overexpression group (Mi-27a) and negative control group (Mi-Con);PLC knockdown cells,Mi-27a-3p knockdown group (Miinhibitor-27a) and negative control group (Mi-inhibitor-Con).The expression of microRNA-27a-3p in each group after transfection was detected by qPCR analysis.MTT assay was used to detect the cell proliferation.Flow cytometry was used to detect the apoptosis and cell cycle.One-way ANOVA was used for multiple comparisons,and t-test was used to compare two groups.Results qPCR results showed that the expression levels of miR27a-3p in L02,HepG2 and PLC increased sequentially,and the relative expression levels were 1.07 ± 0.04,4.81 ± 0.64 and 11.31 ± 0.92,respectively (P ＜ 0.05).MTT assay showed that the cell viability of HepG2 cells transfected with miR-27a-3p overexpression plasrnid was significantly deereased compared with the negative control group (P ＜ 0.05).The apoptosis assay showed that the apoptosis rate of miR-27a-3p overexpression group was higher than the negative control group (P ＜ 0.05).The cell cycle results showed that the proportion of S phase cells in the miR-27a-3p overexpression cell group was significantly lower than the negative control group (P ＜ 0.05).Furthermore,microRNA-27a-3p knockdown validation in PLC cells showed that MTT,apoptosis and cell cycle tests results were opposite to the results of HepG2 overexpression cells,and the differences were statistically significant (P ＜ 0.05).Conclusion miR-27a-3p can significantly inhibit the proliferation of hepatoma cells,promote cell apoptosis,alter the cell cycle distribution,and may become a potential target in hepatocellular carcinoma therapy.
