Elevated Expression of p73 and Its Relation to VEGF and TSP-1 Production in Cervical Carcinoma.
- Author:
Sang Gi SEO
1
;
Sun Kyung LEE
;
Seung Bo KIM
;
Sung Gil CHI
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Kyung Hee University, Seoul, Korea. cish@freechal.com
- Publication Type:Original Article
- Keywords:
p73;
VEGF;
TSP-1;
Cervical carcinoma
- MeSH:
Alleles;
Angiogenesis Inducing Agents;
DNA;
Microvessels;
Polymerase Chain Reaction;
RNA, Messenger;
Thrombospondin 1*;
Uterine Cervical Neoplasms;
Vascular Endothelial Growth Factor A*
- From:Korean Journal of Obstetrics and Gynecology
2006;49(3):599-610
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Measure the over-expression of p73 and analyze as the prognostic as well as angiogenic factor of cervical cancer by comparing the degree of expression of VEGF and TSP-1 by RT-PCR. METHODS: 7 normal and 37 cervical cancer specimens were put through RT-PCR and the expression of p73, VEGF and TSP-1 were measured. After immunohistochemical staining, the number of microvessels was counted. With the level of expression, investigated the relationship with the clinicopathological characteristics and the number of microvessels. RESULTS: 57% of cancer tissues showed abnormally high levels of p73 mRNA. In quantitative genomic DNA PCR, the p73 was over-expressed in the transcription level. Through allotyping with Sty I polymorphism, the over-expression of p73 was due to the transcription activity of the silent allele. In RT-PCR-SSCP analysis of over-expressed specimens, sequence alterations was not seen. In 73%, VEGF was over-expressed while TSP-1 was under-expressed in 35%. There was no association between the number of microvessels with the over-expression of p73 and VEGF, but inversely associated with the under-expression of TSP-1. There was no correlation between the over-expression of p73 and the clinicopathological characteristics. The over-expression of p73 coincided 80% with the over-expression of VEGF, and 40% with the under-expression of TSP-1. CONCLUSION: These data support the expression of p73 was increased in cervical cancer tissues and was associated with the over-expression of the VEGF but not associated with the under-expression of TSP-1. The biological and clinical significance of the over-expression of p73 should be studied further in the future.
