Hepatotoxicity and nephrotoxicity of gallotannin-enriched extract isolated from Galla Rhois in ICR mice.
10.5625/lar.2015.31.3.101
- Author:
Jun GO
1
;
Ji Eun KIM
;
Eun Kyoung KOH
;
Sung Hwa SONG
;
Ji Eun SEUNG
;
Chan Kyu PARK
;
Hyun Ah LEE
;
Hong Sung KIM
;
Jae Ho LEE
;
Beum Soo AN
;
Seung Yun YANG
;
Yong LIM
;
Dae Youn HWANG
Author Information
1. Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea. dyhwang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Galla Rhois;
hepatotoxicity;
nephrotoxicity;
gallotannin;
histopathology
- MeSH:
Administration, Oral;
Alanine Transaminase;
Alkaline Phosphatase;
Animals;
Aspartate Aminotransferases;
Blood Urea Nitrogen;
Body Weight;
Creatinine;
Gallic Acid;
Kidney;
L-Lactate Dehydrogenase;
Lipid Peroxidation;
Liver;
Malondialdehyde;
Mice;
Mice, Inbred ICR*;
Mortality;
No-Observed-Adverse-Effect Level;
Organ Size;
Pathology;
Phenotype
- From:Laboratory Animal Research
2015;31(3):101-110
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate the hepatotoxicity and nephrotoxicity of Galla Rhois (GR) toward the liver and kidney of ICR mice, alterations in related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed after oral administration of 250, 500 and 1,000 mg/kg body weight/day of gallotannin-enriched extract of GR (GEGR) for 14 days. GEGR contained 68.7+/-2.5% of gallotannin, 25.3+/-0.9% of gallic acid and 4.4+/-0.1% of methyl gallate. Also, the level of malondialdehyde (MDA), a marker of lipid peroxidation, was decreased with 19% in the serum of high dose GEGR (HGEGR)-treated mice. The body and organ weight, clinical phenotypes, urine parameters and mice mortality did not differ among GEGR-treated groups and the vehicle-treated group. Furthermore, no significant increase was observed in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and the serum creatinine (Cr) in the GEGR-treated group relative to the vehicle-treated group. Moreover, the specific pathological features induced by most toxic compounds such as CCl4 were not observed upon liver and kidney histological analysis. Overall, the results of the present study suggest that GEGR does not induce any specific toxicity in liver and kidney organs of ICR at doses of 1,000 mg/kg body weight/day, indicating that this is no observed adverse effect level (NOAEL).
