Research advances in application of isobaric tags for relative and absolute quantitation in proteomics of hepatocellular carcinoma
10.3760/cma.j.issn.1007-3418.2016.12.018
- VernacularTitle:相对和绝对定量同位素标记技术在肝细胞癌蛋白质组学中的应用研究进展
- Author:
Yingzi QI
1
;
Lingsheng CHEN
;
Ping XU
Author Information
1. 军事医学科学院放射与辐射医学研究所 北京蛋白质组研究中心蛋白质组学国家重点实验室
- Keywords:
Liver neoplasms;
Proteomics;
Isotope labeling;
Tumor biomarker;
Isobaric tags for relative and absolute quantitation
- From:
Chinese Journal of Hepatology
2016;24(12):952-955
- CountryChina
- Language:Chinese
-
Abstract:
Due to the features of strong heterogeneity,difficult early diagnosis,poor prognosis,and high fatality rate,hepatocellular carcinoma (HCC) has become an important disease which threatens the health of the Chinese population.Accurate early diagnosis is crucial to improving the success rate of liver cancer resection and reducing postoperative recurrence and metastasis,and its core is the screening and validation of biomarkers for early diagnosis.Isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling with amino acids in cell culture are important parts of proteomics technology,and iTRAQ has become the most important technique in quantitative proteomics technology due to its advantages of high throughput,high quantitative accuracy,and no limitation by sample source.This article reviews the research advances in molecular mechanism of the development and progression of HCC and screening of markers,in order to establish a theoretical foundation for in-depth understanding of the molecular mechanisms of the development and progression of HCC and the development of new biomarkers.
