Clinical effect of ultrasound-guided injection of biodegradable poly(lactic-co-glycolic acid)-Fe3O4 in situ implant for magnetic thermal ablation in treatment of nude mice with human liver cancer SMMC-7721 cells
10.3760/cma.j.issn.1007-3418.2016.12.007
- VernacularTitle:超声可视可降解聚乳酸-羟基乙酸共聚物-Fe3O4原位植入物磁感应热消融裸鼠SMMC-7721人肝癌
- Author:
Bing LIANG
1
;
Guoqing ZUO
;
Yuanyi ZHENG
;
Song HE
;
Deyu ZUO
Author Information
1. 400010,重庆医科大学附属第二医院消化内科
- Keywords:
Carcinoma,hepatocellular;
Magnetic hyperthermia;
Implant,biodegradable;
Ultrasound imaging visible
- From:
Chinese Journal of Hepatology
2016;24(12):911-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the Fe3O4-1oaded biodegradable liquid-solid phase inversion poly(lactic-co-glycolic acid) (PLGA) in situ implant for ultrasound-guided injection into nude mouse tumor model,and to investigate its clinical effect in thermomagnetic treatment of nude mice with human liver cancer SMMC-7721 cells in an alternating magnetic field.Methods An in situ implant containing 10％ Fe3O4 was prepared,and 50 μtl Fe3O4-PLGA-NMP gel was injected into the subcutaneous tissue of Kunming mice.The degradation of this material was observed for 2 consecutive months,and the changes in body weight were recorded.HE staining and Prussian blue staining were performed for the heart,liver,spleen,lung,and kidney of Kunming mice.Fresh ex vivo bovine liver was taken and cut into cubes with a dimension of 2 cmu×2 cm×2 cm and then 50 μl Fe3O4-PLGA-NMMP gel was injected;after phase inversion,the cubes ofex vivo bovine liver were heated for 1,2,3,4,and 5 minutes,respectively,and then cut open for observing the range of ablation;HE staining was also performed.Micro-CT scan was performed after ultrasound-guided injection of 50 μl Fe3O4-PLGA gel into the tumors of the nude mice,and then the nude mice were divided into treatment group and control group.The mice in the treatment group were given thermomagnetic treatment for 3 minutes,and tumor growth was observed daily.Results The biodegradation of Fe3O4-PLGA-NMMP implant showed that the subcutaneously injected material was gradually metabolized at 2 weeks after injection and that the nude mice were in good condition.The bovine liver ablation experiment showed that the range of ablation of 50 μl Fe3O4-PLGA implant reached 1.46 ± 0.11 cm.HE staining showed that part of bovine liver had coagulative necrosis.The phase inversion experiment of Fe3O4-PLGA gel showed quick liquid-solid phase inversion of the material after injection into the tumor,and the process of liquid-solid phase inversion could be monitored by ultrasound and CT.The detachment and incrustation of the tumor started at 2 days after treatment,the wound started to heal 15 days later,and the tumor tissue disappeared completely.Conclusion Ultrasound-guided injection of biodegradable Fe3O4-PLGA in situ implant combined with magnetic thermal ablation can effecfively treat human liver cancer SMMC-7721 cells in nude mice.
