Changes in peripheral blood 25-hydroxyvitamin D3, Th17 cells, and CD4+ regulatory T cells and their clinical significance in patients with primary biliary cirrhosis
10.3760/cma.j.issn.1007-3418.2016.11.007
- VernacularTitle:原发性胆汁性肝硬化患者外周血25-羟维生素D3、Th17细胞、CD4+调节性T细胞的变化及其意义
- Author:
Yanming ZHONG
1
;
Xirun WU
;
Qi WANG
;
Meifang YU
;
Ting LU
;
Mingyuan ZHAO
Author Information
1. 粤北人民医院
- Keywords:
Primary biliary cirrhosis;
25-hydroxyvitamin D3;
Th17 cell;
Treg cell
- From:
Chinese Journal of Hepatology
2016;24(11):829-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in peripheral blood 25-hydroxyvitamin D3 [25-(OH)D3],CD4+ regulatory T (Treg) cells,and Th17 cells in patients with primary biliary cirrhosis (PBC) and their mechanism of action in PBC.Methods A total of 22 patients with PBC were enrolled and the male/female ratio was 1∶21,with a mean age of 61± 12 years.There were 7 healthy volunteers matched for age in the normal control group.Electrochemiluminescence immunoassay was used to measure the peripheral blood 25-(OH)D3 level in the PBC group and normal control group,and flow cytometry was used to analyze the changes in Th1 7 cells and CD4+ Treg cells.The t-test,rank sum test,Pearson correlation analysis,or Spearman's rank correlation analysis was used for statistical analysis according to the type of the data.Results The PBC group had a significantly lower serum 25-(OH)D3 levcl than the normal control group (9.49±3.65 vs 27.35±2.35 ng/ml,P ＜ 0.01).Compared with the normal control group,the PBC group had a significantly higher percentage of Th1 7 cells (2.05％±1.17％ vs 0.99％±0.12％,P ＜ 0.01) and a significantly lower percentage of CD4+ Treg cells (2.54％± 1.14％ vs 3.78％±0.51％,P ＜ 0.05);there was a significant difference in Th17/Treg ratio between the PBC group and the normal control group (1.00±0.63 vs 0.26±0.02,P ＜ 0.01).In the PBC group,peripheral blood 25-(OH)D3 was not correlated with Th17 cells or Th17/Treg ratio (r =-0.062 and-0.328,P ＞ 0.05),while it was positively correlated with the percentage ofCD4+ Treg cells (r =0.468,P ＜ 0.05).Conclusion Patients with PBC have significant reductions in peripheral blood 25-(OH)D3 and percentage of CD4+ Treg cells,a significant increase in the percentage ofTh17 cells,and immune unbalance of Th17 cells and CD4+ Treg cells.25-(OH)D3 can upregulate the percentage of CD4+ Treg cells and thus affect the development and progression of PBC,and exogenous vitamin D may improve immune function in PBC patients.
