Protective effect of astragaloside Ⅳ against acute liver failure in experimental mice
10.3760/cma.j.issn.1007-3418.2016.10.011
- VernacularTitle:黄芪甲苷对实验性急性肝衰竭小鼠的保护作用
- Author:
Li LIU
1
;
Shuangjie LI
;
Yu ZHOU
Author Information
1. 311800,浙江省诸暨市人民医院儿科
- Keywords:
Liver failure,acute;
D-gagactosamine;
Lipopolysaccharide;
Astragaloside Ⅳ;
Mice;
Superoxide dismutase;
Malondialdehyde;
Apoptosis
- From:
Chinese Journal of Hepatology
2016;24(10):772-777
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical effect of astragaloside Ⅳ in the early treatment of mice with acute liver failure and possible mechanisms.Methods A mouse model of acute liver failure induced by D-galactosamine/lipopolysaccharide (D-GalN/LPS) was established,and the mice were given astragaloside Ⅳ at different doses.The survival rate of mice,alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels,liver histopathological changes,apoptosis of hepatocytes,and the expression of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in each group.The least significant difference test was used for data with homogeneity of variances,the Dunnett's T3 test was used for data with heterogeneity of variance,and the Kaplan-Meier method was used for survival analysis.Results Compared with the model group,the highdose astragaloside Ⅳ group had a significant increase in the 48-hour survival rate [60％ (9/15) vs 13.3％ (2/15),P ＜ 0.05],significant reductions in the serum ALT and AST levels (P ＜ 0.01),and significant reductions in liver histopathological indices and the degree of apoptosis of hepatocytes (P ＜ 0.01),as well as a significant reduction in the content of MDA in liver homogenate (P ＜ 0.01) and a significant increase in the activity of SOD (P ＜ 0.05).Conclusion High-dose astragaloside Ⅳ has a significant protective effect against D-GalN/LPS-induced acute liver injury in mice,and its mechanisms may be associated with its effects against cell apoptosis and oxidative damage.
