Expression and mutational analysis of TGF-beta/Smads signaling in human cervical cancers.
10.3802/jgo.2009.20.2.117
- Author:
Kyung Do KI
1
;
Seo Yun TONG
;
Chu Yeop HUH
;
Jong Min LEE
;
Seon Kyung LEE
;
Sung Gil CHI
Author Information
1. Department of Obstetrics and Gynecology, East-West Neo Medical Center, Kyung Hee University, Seoul, Korea. diners99@naver.com
- Publication Type:Original Article
- Keywords:
TGF-beta/Smads;
Cervical cancer;
Expression
- MeSH:
Humans;
Polymerase Chain Reaction;
RNA, Messenger;
Transforming Growth Factor beta1;
Uterine Cervical Neoplasms
- From:Journal of Gynecologic Oncology
2009;20(2):117-121
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To define the molecular basis of TGF-beta1 function in cervical carcinogenesis, we explored the expression and mutational status of TGF-beta1, TGF-beta1 receptors, and Smads, the regulators of the TGF-beta1 signaling pathway, in human cervical cancers. METHODS: Expression of TGF-beta1, TGF-beta1 receptors, and Smads transcripts were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR), and sequence alteration was analyzed using RT-PCR-single-strand conformation polymorphism (SSCP) analysis. Genomic levels of TGF-beta1, TGF-beta1 receptors and Smads was also measured by quantitative genomic PCR. RESULTS: Abnormal overexpression of TGF-beta1 and abnormal reduction of type II TGF-beta1 receptor were identified in 36% (18 of 50) and 20% (10 of 50) of cervical cancer tissues, respectively. 22% (11 of 50) in Smad2 and 14% (7 of 50) in Smad4 revealed tumor specific mRNA reduction less than a half of normal means. In addition, no evidence for sequence alterations of the gene was found by RT-PCR-SSCP analysis. CONCLUSION: Our study demonstrates that disruption of TGF-beta/Smad signaling pathway exist in human cervical cancer, suggesting that abnormal expressions of the member of TGF-beta/Smad signaling pathway might contribute to the malignant progression of human cervical tumors via suppressing the tumor suppression function of TGF-beta1 1's tumor suppression function.
