Screening of serum biomarkers by isobaric tags for relative and absolute quantitation in patients with HBV-related acute-on-chronic liver failure
10.3760/cma.j.issn.1007-3418.2016.08.005
- VernacularTitle:基于同位素标记相对和绝对定量技术筛选乙型肝炎慢加急性肝衰竭患者的血清生物标志物
- Author:
Huimin LIU
1
;
Fangyuan GAO
;
Hao YU
;
Peipei MENG
;
Yuyong JIANG
;
Xianbo WANG
Author Information
1. 100015,首都医科大学附属北京地坛医院中西医结合中心
- Keywords:
Liver failure;
Hepatitis B,chronic;
Biomarkers;
Isobaric tags
- From:
Chinese Journal of Hepatology
2016;24(8):580-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the screening of serum biomarkers in patients with HBV-related acute-on-chronic liver failure (HBV-ACLF) by isobaric tags for relative and absolute quantitation (iTRAQ).Methods Gel electrophoresis was used to isolate and remove high-abundant proteins.Each group ofpeptides was labeled by the iTRAQ reagents and then tested with an UltiMateTM 3000 nanoliter high-performance liquid chromatograph,and a Q-Exactive tandem mass spectrometer.The Protein Discovery software was used to analyze mass spectrometry data and perform bioinformatic analysis for differentially expressed proteins.Results Ten samples each were included in the HBV-ACLF group and the chronic hepatitis B (CHB) group,and six samples each were included in the HBV-ACLF survival group and the HBV-ACLF death group.Compared with the CHB group,the HBV-ACLF group had 43 differentially expressed proteins,among which 34 were downregulated and 9 were upregulated.Compared with the HBV-ACLF survival group,the HBV-ACLF death group had 33 differentially expressed proteins,among which 18 were upregulated and 15 were downregulated.Conclusion Keratin,α1-acid glycoprotein,and zinc-α2-glycoprotein identified in the serum may be used as potential biomarkers for predicting the prognosis of patients with HBV-ACLF.
