Expression profiles of long non-coding RNAs in human liver cell line LO2 with stable expression of hepatitis Bx gene
10.3760/cma.j.issn.1007-3418.2016.06.005
- VernacularTitle:稳定表达HBx的人肝细胞株L02长链非编码RNA表达谱分析
- Author:
Xuyuan KUANG
1
;
Ning LI
;
Yongming FU
;
Jia LI
;
Xuegong FAN
Author Information
1. 中南大学湘雅医院感染科 病毒性肝炎湖南省重点实验室
- Keywords:
Carcinoma,hepatocellular;
Hepatitis B virus;
HBx gene;
Long non-coding RNA;
Microarray
- From:
Chinese Journal of Hepatology
2016;24(6):417-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression profiles of long non-coding RNAs (lncRNAs) in human liver cell line LO2 with stable expression of hepatitis B x (HBx) gene,and to screen out the lncRNAs which play an important role in HBV-related liver cancer.Methods The lncRNA microarray was used to establish the differential expression profiles of lncRNAs,and the methods such as scatter plots and cluster analysis were used to obtain the HBx-related lncRNAs with differential expression.The qRT-PCR was used to verify some lncRNAs with differential expression.The t-test was used to compare the expression of lncRNAs between the two microarray groups,and hierarchical cluster analysis was used for the original data of lncRNAs with differential expression.Results Compared with the control group transfected with blank plasmids (L02/pcDNA3.0),LO2/HBx cells had 323 lncRNAs with ＞ 2-fold upregulation and 421 lncRNAs whose expression was reduced by more than 50％ (P ＜ 0.05).The results of qRT-PCR verified 4 upregulated lncRNAs (TCONS 00006195,ENST00000557524,NR_037597,and ENST00000539975) and 3 downregulated lncRNAs (ENST00000508424,ENST00000447433,and uc001lva.4),which were consistent with the results of microassay.Conclusion HBx-related lncRNAs are successfully screened out,which lays a foundation for further investigation of the role of lncRNAs in the pathogenesis of liver cancer.
