Association between interleukin-18 gene polymorphisms and hepatocellular carcinoma caused by hepatitis B virus
10.3760/cma.j.issn.1007-3418.2016.05.008
- VernacularTitle:白细胞介素18基因多态性与HBV所致肝细胞肝癌的相关性
- Author:
Qingxiu ZHANG
1
;
Yunqing YAO
;
Shiliang LI
;
Qin LONG
Author Information
1. 400016,重庆医科大学附属第一医院感染科
- Keywords:
Carcinoma,hepatocellular;
Hepatitis B virus;
Interleukin-18;
Gene polymorphism
- From:
Chinese Journal of Hepatology
2016;24(5):352-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between the single nucleotide polymorphisms (SNPs) IL-18-137G/C (rs187238) and IL-18-607A/C (rs1946518) in interleukin-18 (IL-18) gene and hepatocellular carcinoma (HCC) caused by the hepatitis B virus (HBV).Methods The subjects were divided into HBV-related HCC group (109 patients),chronic HBV infection group (113 patients),and healthy control group (127 patients).The polymerase chain reaction-ligase detection reaction (PCR-LDR) was used to determine the alleles and genotypes of the two SNPs IL-18-137G/C and IL-18-607A/C.The t-test and chi-square test were used for baseline data.The chi-square test was used to investigate the differences in genotype and allele frequencies across the three groups.Non-conditional logistic regression analysis was used to compare the odds ratios (ORs) and 95％ confidence intervals (CIs) for different genotypes/alleles in predicting the risk ofHBV-related HCC.Results The HBV-related HCC group showed significantly higher AA genotype and A allele frequencies of the SNP IL-18-607A/C than the healthy control group (AA genotype frequency:29.4％ vs 18.1％,x2 =4.152,P ＜ 0.05;A allele frequency:54.6％ vs 44.1％,5.169,P ＜ 0.05),which were positively correlated with the risk of HBV-related HCC (AA genotype frequency:OR =1.879,95％ CI:1.020-3.464;A allele frequency:OR =1.524,95％ CI:1.059-2.193).The chronic HBV infection group had a significantly higher A allele frequency of the SNP IL-18-607A/C than the healthy control group (54.0％ vs 44.1％,x2 =4.680,P ＜ 0.05),which was positively correlated with the risk of chronic HBV infection (OR =1.487,95％ CI:1.037-2.132).The genotype and allele frequencies of the SNP IL-18-607A/C showed no significant differences between the HBV-related HCC group and the chronic HBV infection group (P ＞ 0.05).The genotype and allele frequencies of the SNP IL-18-137G/C showed no significant differences between any two groups of the three groups (P ＞ 0.05).Conclusion The AA genotype and A allele frequencies of the SNP IL-18-607A/C are positively correlated with the morbidity of HBV-related HCC,and the A allele frequency of the SNP IL-18-607A/C is positively correlated with the morbidity of chronic HBV infection.
