Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis.
10.14405/kjvr.2017.57.2.117
- Author:
Danil KIM
1
;
Eun Kyung KIM
;
Won Jin SEONG
;
Younghye RO
;
Dae Sung KO
;
Nam Hyung KIM
;
Jae Hong KIM
;
Hyuk Joon KWON
Author Information
1. Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. kwonhj01@snu.ac.kr
- Publication Type:Original Article
- Keywords:
antibiotic resistance;
bovine mastitis;
microbial pathogens;
microbiome;
raw milk
- MeSH:
Agriculture;
Ampicillin;
Animals;
Cattle;
Chickens;
Drug Resistance, Microbial;
Ducks;
Egg White*;
Enterococcus faecalis;
Escherichia coli;
Female;
Genes, rRNA*;
Mastitis;
Mastitis, Bovine*;
Methods;
Microbiota*;
Milk;
Muramidase;
Norfloxacin;
Ovum*;
Staphylococcus aureus
- From:Korean Journal of Veterinary Research
2017;57(2):117-126
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.
