Oxymatrine inhibits target cell infection in the HCVcc system
10.3760/cma.j.issn.1007-3418.2016.01.008
- VernacularTitle:氧化苦参碱抑制体外细胞培养模型HCV感染靶细胞研究
- Author:
Na CHEN
1
;
Yehong LIU
;
Xiaojing LIU
;
Yunru CHEN
;
Yonghong GUO
;
Min LIU
Author Information
1. 710061,西安交通大学第一附属医院感染科
- Keywords:
Hepatitis C virus;
Infection;
HCV grown in cell culture;
Oxymatrine
- From:
Chinese Journal of Hepatology
2016;24(1):40-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To validate the antiviral effect of oxymatrine against hepatitis C virus (HCV) in the construction of the cell culture-based infectious virus system HCVcc.Methods An HCVcc system was established by infecting Huh7.5.1 cells with the J6 adapted virus (J6cc).Cells of the HCVcc system were then treated with different concentrations of oxymatrine for 24 h,48 h and 72 h,or left untreated (controls).MTT assay was used to detect effects on proliferation.Real-time quantitative PCR was used to detect effects on HCV mRNA expression level,and immunofluorescence was used to detect effects on HCV protein expression.Data were expressed as mean±standard deviation,and statistically assessed using one-way ANOVA and one-sample t-test.Results Treatment with 2,4,8 and 12 mg/mL of oxymatrine for 24 h inhibited proliferation of the J6cc-infected cells by 8.4％,15.2％,29.6％ and 48.6％ respectively,48 h treatment inhibited by 14.3％,25.7％,46.1％ and 66.4％ respectively,and 72 h treatment inhibited by 36.5％,46.6％,70.6％ and 85.4％ respectively.Thus,the effects of oxymatrine were time-and dose-dependent (P ＜ 0.05).The mRNA expression level in the oxymatrine-treated cells of the HCVcc system was 0.59 ± 0.12,which was significantly lower than that in the control cells (P ＜0.05).Moreover,as the oxymatrine concentration increased from 2 mg/mL to 12 mg/mL,the expression levels of HCV proteins also showed a decreasing trend.Conclusion We successfully constructed a J6cc infection HCVcc system and verified the antiviral effect of oxymatrine against HCV.
