Construction of the recombinant retrovirus vector of HBV-S gene and it's expression in eukaryotic cells

VernacularTitle:乙型肝炎病毒S基因重组逆转录病毒载体的构建及其在真核细胞中表达
Author: Zhi ZHOU ¹ ; Dingfeng ZHANG ; Hong REN
Author Information

1. 重庆医科大学
Keywords: Hepatitis B virus; Gene recombinant; Eukaryotic expression
From: Chinese Journal of Hepatology 2000;8(5):296-298
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effectiveness of recombinant retrovirus vector in gene therapy. Methods: The retroviral vector PLXSN-S was constructed and transferred into PA317 by means of electroporation, then HepG2, P815, and EL4 cells were infected with the pseuovirus produced from PA317, which highly expressed HBsAg. HBsAg expression was tested by RT-PCR and ELISA. Results: HBsAg was expressed variously in the eukaryotic cells mentioned above. HBsAg (A value) of the cell supernatants (48h) were 0.92, 0.09, 0.47, respectively. Conclusion: The vector used in this study is an effective one to carry genes of interest to target cells and it may be useful in the test for gene therapy.