Experimental Study on the Regulation of AMPK Protein and mRNA Expression in Mouse Endometrium on Embryo Implantation
10.3969/j.issn.1671-7414.2024.06.007
- VernacularTitle:小鼠子宫内膜中AMPK蛋白及mRNA表达对胚胎着床调控作用的实验研究
- Author:
Kao MENG
1
;
Juan MA
Author Information
1. 安徽医科大学附属宿州医院/宿州市立医院生殖医学科,安徽 宿州 234000
- Keywords:
embryo implantation;
endometrium;
AMP-activated protein kinase;
early pregnant mice
- From:
Journal of Modern Laboratory Medicine
2024;39(6):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of AMP-activated protein kinase(AMPK)protein and mRNA expression in endometrium of pregnant mice on embryo implantation.Methods ICR mice were used to establish 1~8 days normal pregnancy mice and 1~5 days pseudopregnancy mice models,which were divided into negative control(NC)group and AMPK inhibitor(Dorsomorphin)group.Immunohistochemical staining was used to detect the expression position of AMPK protein in the endometrium of mice.Reverse transcripatase quantitative polymerase chain reaction(RT-qPCR)was used to detect the AMPK in the endometrium of mice,and the related indicators of implantation in the uterus including the mRNA levels of AMPK,mucin1(Muc1),homeobox A10(Hoxa10)and cardiac neural ridge derivative expression protein 2(Hand2).Western blotting was applied to detect AMPK protein levels in endometrium and count the number of embryonic blastocysts in pregnant mice.Results The expression of AMPK protein and mRNA were gradually increased from day 1st to day 5th of pregnancy(F=683.50,678.20,all P<0.05),and mainly expressed in endometrial lumen cells and glandular epithelial cells.Compared with day 5th of gestation,the expressions of AMPK protein and mRNA in uterus of micedecroused on the 6th day of gestation(t=25.98,68.41,all P<0.05),and the expression was mainly in the primary decidualizing zone(PDZ)and the secondary decidualizing zone(SDZ).The expressions of AMPK protein and mRNA in endometrium were gradually increased on the 6th to 8th day of pregnancy(t=86.00,54.05;29.96,127.60,all P<0.05).Compared with 1st day of pseudogestation,the expression of AMPK mRNA and protein decreased on days 2~5 of pseudogestation(t=38.97,30.45,33.00,13.98;22.71,26.90,53.81,65.00,all P<0.05),,and mainly expressed in gland and luminal opithelial cells.Compared with NC group,Muc1 mRNA(5.97±0.32 vs 1.10±0.15)expression increased,(18.20±3.19),Dorsomarphin group(9.40±2.41)reuced the mean number of blastoblasts(t=8.73,P<0.05),HoxA10(0.20±0.10 vs 1.03±0.06)and Hand2(0.23±0.15 vs 0.97±0.12)mRNA expression decreased at the implantation site(t=32.15,3.46,2.65,all P<0.05).Conclusion AMPK in mouse endometrial tissue plays an important role in embryo implantation,which inhibits AMPK expression and will be detrimental to implantation.