Diagnostic Value of Combined Detection of Serum PDK4,DECR1 and MMP1 in Diabetes Cardiomyopathy
10.3969/j.issn.1671-7414.2024.05.024
- VernacularTitle:糖尿病性心肌病患者血清PDK4,DECR1和MMP1表达水平及临床价值研究
- Author:
Meijuan MA
1
;
Huiqin SONG
;
Guoan ZHANG
;
Xiaoyan HUANG
Author Information
1. 陕西省人民医院西院体检中心,西安 710068
- Keywords:
diabetes cardiomyopathy;
pyruvate dehydrogenase kinase isoenzyme 4;
2,4-dienoyl coenzyme A reductase 1;
matrix metalloproteinase 1
- From:
Journal of Modern Laboratory Medicine
2024;39(5):130-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of combined detection of serum pyruvate dehydrogenase kinase isoenzyme 4(PDK4),2,4-dienoyl coenzyme A reductase 1(DECR1)and matrix metalloproteinase 1(MMP1)in the diagnosis,clinical grading and prognosis of diabetes cardiomyopathy(DCM).Methods A sum of 26 patients with diabetes cardiomyopathy(DCM group)and 120 patients with diabetes non cardiomyopathy(control group)who were admitted to Shaanxi Provincial People's Hospital from October 2021 to October 2023 were selected.The expression levels of PDK4,DECR1 and MMP1 proteins in serum were measured by enzyme-linked immunosorbent assay(ELISA)to evaluate the diagnostic value of these three detection indicators in DCM.Results Compared with the Control group,the levels of serum PDK4(131.38±10.20 pg/ml vs 82.69±8.17 pg/ml),DECR1(152.06±12.57 pg/ml vs 86.14±9.55 pg/ml)and MMP1(40.27±4.02 μg/ml vs 17.77±0.98 μg/ml)protein in the diabetes cardiomyopathy(DCM)group were significantly higher,and the differences were statistically significant(t=36.24,47.63,12.29,all P＜0.001).In the DCM group,the protein expression levels of serum PDK4,DECR1 and MMP1 were correlated with NYHA cardiac function grading,while the protein expression levels were significantly increased with the grade increasing,and the differences between the groups were statistically significant(F=24.12,30.04,12.66,all P＜0.001).In the DCM group,compared with the mild group,the expression levels of serum PDK4(164.92±1.35pg/ml vs 122.48±8.78pg/ml),DECR1(192.17±9.11pg/ml vs 124.36±10.83pg/ml)and MMP1(84.44±7.38 μg/ml vs 39.41±3.05 μ g/ml)proteins were significantly increased in patients with moderate to severe illness,and the differences were statistically significant(t=26.33,47.12,15.41,all P＜0.001).The accuracy(x2=18.23,21.37,22.07),specificity(x2=9.72,13.43,15.12)and sensitivity(x2=12.07,16.07,17.55)of serum PDK4,DECR1 and MMP1 were significantly higher than those of single Test(all P＜0.05),the results of ROC curve analysis showed that the AUC of combined detection was 0.955,which was significantly higher than that of single detection(Z=16.67,17.09,20.44,all P＜0.05).Conclusion Serum PDK4,DECR1 and MMP1 are related to the diagnosis,clinical grading and prognosis of diabetes cardiomyopathy.The combined detection of the three is helpful to the differential diagnosis of diabetes cardiomyopathy.
