Detection and Clinical Significance of DAPL1 and MLH1 Gene Methylation in Bronchoalveolar Lavage Fluid of Lung Cancer Patients
10.3969/j.issn.1671-7414.2024.05.023
- VernacularTitle:肺癌患者支气管肺泡灌洗液中DAPL1和MLH1基因甲基化水平检测及其临床意义研究
- Author:
Shilong LUO
1
;
Zhiqiang LI
;
Xiang LI
Author Information
1. 蒙城县第二人民医院肿瘤内科,安徽蒙城 233500
- Keywords:
lung cancer;
bronchoalveolar lavage fluid;
death-associated protein kinase like 1;
mismatch repair gene;
gene methylation
- From:
Journal of Modern Laboratory Medicine
2024;39(5):125-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of death-associated protein kinase like 1(DAPL1)and mismatch repair gene(Mut L homologue 1,MLH1)methylation level in bronchoalveolar lavage fluid(BALF)of patients with lung cancer,and further investigate the diagnostic value of gene methylation in early lung cancer and its relationship with clinicopathological features.Methods A total of 142 patients with suspected early stage lung cancer admitted the Second People's Hospital of Mengcheng County from January 2022 to January 2024 were retrospectively selected and divided into lung cancer group(n=82)and lung benign lesion group(n=60)according to the final pathological results.Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the methylation levels of DAPL1 and MLH1 in BALF samples.The clinical diagnostic value of DAPL1 and MLH1 methylation in early stage lung cancer and its relationship with clinicopathological features of lung cancer patients were analyzed.Results The methylation levels of DAPL1 and MLH1 gene in BALF in lung cancer group were 53.66％(44/82)and 56.10％(46/82),respectively,which were significantly higher than those in benign disease group[11.67％(7/60)and 18.33％(11/60)],and the difference was statistically significant(x2=56.544,20.565,all P＜0.05).The sensitivity of DAPL1 andMLH1 gene methylation in the diagnosis of early lung cancer was 53.66％(44/82)and 56.10％(46/82),the specificity was 88.33％(53/60)and 81.67％(49/60),and the accuracy were 68.31％(97/142)and 66.90％(95/142),respectively.The sensitivity and accuracy of DAPL1 methylation combined with MLH1 methylation in the diagnosis of early lung cancer was 86.59％(71/82),and 85.92％(122/142),respectively,both of which were higher than that of a single index(Z=24.411,16.450,all P＜0.05).The methylation levels of DAPL1 and MLH1 genes in BALF of lung cancer patients were closely correlated with clinical stage,smoking history and lymph node metastasis(x2=5.493,13.083;8.167,6.946;9.303,4.523,all P＜0.05).Spearman correlation showed that DAPL1 and MLH1 gene methylation were positively correlated with clinical stage,smoking history and lymph node metastasis in patients with lung cancer(r=0.523,0.602;0.548,0.498;0.630,0.524,all P＜0.05).Conclusion The methylation of DAPL1 and MLH1 genes in BALF has high clinical diagnostic value for early lung cancer,and the methylation levels of both genes are related to the disease progression and smoking history of lung cancer patients.
