Study on the Relationship between the Expression of Serum miR-455-3p and miR-141-3p Levels and the Severity of Disease in Patients with Acute Pancreatitis
10.3969/j.issn.1671-7414.2024.05.008
- VernacularTitle:急性胰腺炎患者血清miR-455-3p,miR-141-3p水平表达与病情严重程度的关系研究
- Author:
Qiliang GAO
1
;
Xingjian MAO
;
Gang WU
Author Information
1. 都江堰市人民医院消化内科,四川都江堰 611830
- Keywords:
acute pancreatitis;
miR-455-3p;
miR-141-3p
- From:
Journal of Modern Laboratory Medicine
2024;39(5):41-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the expression of miR-455-3p and miR-141-3p levels in serum and the severity of acute pancreatitis(AP)in patients.Methods A total of 133 AP patients(AP group)who visited the People's Hospital of Dujiangyan from November 2021 to November 2023 were regarded as the study subjects.According to the severity of disease,AP patients were separated into a severe group(n=43)and a mild group(n=90).During the same period,135 healthy individuals who underwent physical examination in the hospital were regarded as the control group.Real-time fluorescence quantitative PCR method was applied to detect serum levels of miR-455-3p and miR-141-3p.Spearman correlation analysis was applied to analyze the correlation between serum miR-455-3p and miR-141-3p levels and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)and Ranson scores.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum miR-455-3p and miR-141-3p levels for severe AP.Multivariate logistic regression was applied to analyze the factors affecting the occurrence of severe AP.Results The serum miR-455-3p level(0.61±0.13)in the AP group was lower than that in the control group(1.12±0.21),while the miR-141-3p level(1.37±0.11)was higher than that in the control group(1.04±0.15),with significant differences(t=23.863,20.513,all P＜0.05).The serum miR-455-3p level(0.53±0.10)in the severe group was lower than that in the mild group(0.64±0.12),while the miR-141-3p level(1.51±0.14)was higher than that in the mild group(1.30±0.15),with significant differences(t=6.056,7.713,all P＜0.05).The serum amylase(AMS),lipase(LPS)levels,APACHE Ⅱ and Ranson scores in the severe group were higher than those in the mild group,and the differences were significant(t=2.227,2.290,18.267,11.259,all P＜0.05).Spearman correlation analysis results showed that the serum miR-455-3p of AP patients was negatively correlated with APACHEⅡ and Ranson scores(r=-0.702,-0.783,all P＜0.05),while miR-141-3p was positively correlated with APACHEⅡ and Ranson scores(r=0.787,0.734,all P＜0.05).ROC curve results showed that the AUCs of serum miR-455-3p and miR-141-3p levels alone and combination of severe AP were 0.848,0.822 and 0.919,respectively.And the combination detection was superior to single detection(Z=3.081,2.524,all P＜0.05).Multivariate Logistic regression analysis showed that miR-455-3p was a protective factor affecting severe AP,while miR-141-3p was a risk factor affecting severe AP(P＜0.05).Conclusion The decrease of serum miR-455-3p and the increase of miR-141-3p in AP patients are closely related to the severity of disease.
