Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection
10.3969/j.issn.1671-7414.2024.03.034
- VernacularTitle:CRISPR-Cas13a系统在病原体检测应用中的最新研究进展
- Author:
Qingsong LI
1
;
Congping ZHAO
;
Jing LIU
;
Jun YANG
;
Siyu YAN
;
Yinhe ZHANG
Author Information
1. 西南医科大学公共卫生学院,四川泸州 646000
- Keywords:
clustered regularly interspaced short palindromic repeats/CRISPR-associated 13a systems;
pathogen;
nucleic acid detection
- From:
Journal of Modern Laboratory Medicine
2024;39(3):199-204
- CountryChina
- Language:Chinese
-
Abstract:
As a new generation of molecular diagnostic tools emerged in recent years,the clustered regularly interspaced short palindromic repeats/CRISPR-associated(CRISPR-Cas)systems have been widely used in gene editing,nucleic acid detection,and other fields.Due to its high specificity,high sensitivity,without expensive equipment,simple operation,and inexpensive characteristics in nucleic acid detection,the Cas13a subtype has shown great potential in the field of pathogen detection.This article reviews the mechanism of action of CRISPR-Cas13a,its application in pathogen detection,and related detection methods,and looks forward to the application prospects of Cas13a.To facilitate future investigations on the CRISPR-Cas13a systems and their potential in pathogen detection,this study aims to offer inspiration and serve as a valuable reference.
