Effects of TNF-α on IL-8 mRNA Expression in Endothelial Cells
- VernacularTitle:TNF-α对内皮细胞白介素-8基因表达的影响
- Author:
Lizhi WU
1
;
Huaiqing CHEN
;
Youqin CHEN
;
Boyao WANG
Author Information
1. West China University of Me dical Sciences
- From:
Journal of Biomedical Engineering
2001;18(1):105-107
- CountryChina
- Language:Chinese
-
Abstract:
As a kind of chemokine, IL-8 plays an important role in inflammatory reaction. Its expression in endothelial cells is regulated by a variety of cytokines. In this study, after incubating cultured human umb ilical vein endothelial cells(HUVECs) with TNF-α for different time, we employed RT-PCR to assay IL-8 mRNA expression and immunocytochemical stain ing to detect NF-κB activation in HUVECs. The results revealed:(1)IL-8 mRNA e xpressed little in untreated HUVECs; after HUVECs was pre-incubated with TNF-α for 1 hour, IL-8 mRNA expression increased greatly, and it increased further after pre-incubated for 3 hours; howerver, when HUVECs was pre-incubated with TNF-α f or 6 hours, IL-8 mRNA expression decreased, and it almost decreased to basal l evel when pre-incubated for 9 hours; (2)the immunoreactivity of NF-κB p65 in the nuclei of untreated HUVECs was negative; it became positive in the nuclei of HUVECs pre-incubated with TNF-α for 1 hour and 3 hours; it became weak po s itive in the nuclei of HUVECs pre-incubated with TNF-α for 6 hours, and after 9 hours, pre-incubation, the immunoreactivity of NF-κB p65 in the nuclei of HUVECs was negative. These results implied that TNF-α was capable of inducing IL-8 gene expression and activation NF-κB. and Their time courses were similar, thus suggesting that the induction of IL-8 gene expression by TNF-α is probably due to the activation of NF-κB.
