Large-Capacity Expanded Cytoline-induced Killer Cells and Its Cytotoxic Activity
- VernacularTitle:细胞因子诱导杀伤(CIK)细胞的大容量扩增与杀伤活性观察
- Author:
Yongjin SHI
1
;
Jiren YU
;
Xinan CEN
;
Ping ZHU
;
Mingxin MA
Author Information
1. Beijing University
- From:
Journal of Biomedical Engineering
2001;18(1):94-96
- CountryChina
- Language:Chinese
-
Abstract:
This study was conducted to estabilish the large -capacity culture methd of cytokine-induced killer(CIK) cells for clinical th erapy and assess its effect on the fuction of cell-mediated immunity foll owing autologous CIK cells reinfusion. Autologus CIK cells were expanded in 1000 ml cluture-bag and reinfused back. The MTT method was used to test the cytoto xicactivity of CIK cells before and after reinfusion. The results showed that the total amount of autologous CIK cells reinfusion exceeded 1.6×1010with the use of the culture method of large-capacity. The PBMNC from patients treated by CIK cells showed significant increase in cytotoxic activity, no side effects were observed, and therefore the large-capacity cultu re method of CIK cells is a simle and safe therapy for treating the minimum residue of diseases.
