Fusion Expression of Cecropin X Including the Cleavage of FXa in Escherichia coli

VernacularTitle:含有FXa切割位点的抗菌肽X在大肠杆菌中的融合表达
Author: Liu-Di YUAN ¹ ; Fei DOU ; Yu-Pu LIANG ; Wei XIE ; Fang WANG ; Shuang-Quan ZHANG ; Zhu-Ying DAI
Author Information

1. Nanjing University
Keywords: Cecropin X; fusion expression; Factor Xa
From: Chinese Journal of Biotechnology 2000;16(3):411-414
CountryChina
Language:Chinese
Abstract: PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X, then the gene was cloned into the expression vector pGEX-KG, and was highly expressed in E. coli BL21 by IPTG induction. The fusion protein was purified by affinity-chromatography and was cleaved by Factor Xa. Cecropin X with antibacterial activity was obtained after purified by ion-exchange chromatography.