High-level Expression of BYDV GAV Coat Protein Gene in Escherichia coli
10.3321/j.issn:1000-3061.2000.03.024
- VernacularTitle:大麦黄矮病毒GAV株系外壳蛋白基因在大肠杆菌中的高效表达
- Author:
Sheng-Jun CHANG
1
;
Zhan-Hong MA
;
Xi-Feng WANG
;
Li LI
;
Guang-He ZHOU
Author Information
1. The Institute of Plant Protection, The Chinese Agricultural Academy of Science, Beijing 100094
- Keywords:
BYDV GAV;
coat protein gene;
expression
- From:
Chinese Journal of Biotechnology
2000;16(3):400-402
- CountryChina
- Language:Chinese
-
Abstract:
The IPTG-inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E. coli BL21 (DE3). High-level expression of the specific protein was achieved by IPTG induction. The results of SDS-PAGE and Western blottong show that the expression product which accumulates 19.5 % of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET-5a.
