Study on Continuous Synthesis of Galacto-oligosaccharide by Immobilized Bacillus stearothermophilus
10.3321/j.issn:1000-3061.2000.03.022
- VernacularTitle:固定化嗜热脂肪芽孢杆菌连续合成半乳糖寡糖的研究
- Author:
Dong-Zhi WEI
1
;
Xiao-Lan WANG
;
Shao-Xin CHEN
;
Jun-Tang YU
Author Information
1. 华东理工大学
- Keywords:
Galacto-oligosaccharide;
Bacillus stearothermophilus;
β-galactosidase;
immobilized cell;
fibrous bed bioreactor
- From:
Chinese Journal of Biotechnology
2000;16(3):392-395
- CountryChina
- Language:Chinese
-
Abstract:
The galacto-oligosaccharide was synthesized continuously by immobilized Bacillus stearothermophilu producing β-galactosidase in fibrous bed reactor. The effect of substrate concentration, pH, reaction temperature and retention time on production of GOS was investigated. The optimal reaction conditions were determined. Substrate concentration were 450 g/L;Reaction temperature was 55℃; pH was 7.0; Residience time was 100 min. The product yield reached up to 50.7 %. GOS synthesis was promoted by feeding 1.5 % D-glactose after 24h. The immobilized cell reactor can work stably for 120h.
