Sdudies on Simultaneous Production of Chitosanase and Chitosan Degradation in situ with Trichoderma reesei in Convoluted Fibrous Bed Bioreactor
10.3321/j.issn:1000-3061.2000.03.017
- VernacularTitle:螺旋纤维床固定化生物反应器同时产酶降解壳聚糖的研究
- Author:
Mian-Bin WU
1
;
Li-Ming XIA
;
Pei-Lin CEN
Author Information
1. 浙江大学
- Keywords:
Trichoderma reesei;
convoluted fibrous bed bioreactor;
chitosanase production and hydrolysis of chitosan
- From:
Chinese Journal of Biotechnology
2000;16(3):368-372
- CountryChina
- Language:Chinese
-
Abstract:
The cells of Trichoderma reesei were immobilizxed on a roll of porous polyurethane foam sheet and packed in a bubble column bioreactor for simultaneous production of chitosanase and degradation of chitosan in situ. The average degree of polymerization could be regulated by reaction time. Under the repeated-batch process with 2 % soluble chitosan at pH4.8,28℃ , the activity of chitosanase for each batch was above 0.15 IU/mL, the average yield of reducing sugar as D-glucosamine reached 73%. The novel immobilized bioreactor system run stably and effectively in the successive 10 batches lasting 30 days without notable change in the activity and productivity.
