Application of Intermittent-feeding of Growth-limiting Nutrients in Suspension Culture of Insect Cells(Sf21)
- VernacularTitle:昆虫细胞(Sf21)悬浮培养过程中生长限制性基质间歇补加技术的应用
- Author:
Jiao ZHAO
1
;
Wen-Song TAN
;
Yan ZHOU
;
Li ZHOU
;
Jun-Tang YU
Author Information
1. 华东理工大学
- Keywords:
Insect cells;
suspension culture;
growth-limiting nutrients;
intermittent- feeding;
growth
- From:
Chinese Journal of Biotechnology
2000;16(3):357-362
- CountryChina
- Language:Chinese
-
Abstract:
On the basis of the growth and metabolism behavior inherent in suspended Sf21 insect cells,the intermittent feeding of growth-limiting nutrients(glucose and protein hydrolysates)was employed in the regulation of cellular growth during the cultivation by using the residual glucose concentration as a reference point. It was shown that as compared with the batch cultivation, the intermittent-feeding of growth-limiting nutrients effectively prolonged the growth and stationary phase for Sf21 insect cells grown in two representative culture medium(TC-100 and IPL-41). The maximum cell density was increased from 3.0×106 cells/ml to 6.5×106 cells/mL in TC-100 medium, and in IPL-41 medium, the maximum cell density was increased from 7.05×106 cells/mL to 9.0×106 cells/mL. As the defined nutrient solution was used for feeding in lieu of the complicated and expensive base medium, the technique would find prospects in large scale high-density cultivation of insect cells.
