Expression of Prourokinase in Different Mammalian Cells

VernacularTitle:不同哺乳动物细胞表达尿激酶原的研究
Author: Feng QIAN ¹ ; Cheng-Zu XIAO ; Li-Hua GAO ; Zheng-Guang ZHANG ; Zhi-Xia GUO ; Wei-Yuan YU
Author Information

1. Institute of Biotechnology
Keywords: Prourokinase; Namalwa cells; Vero cells; Sp2/0 cells; stability of protein
From: Chinese Journal of Biotechnology 2000;16(3):349-352
CountryChina
Language:Chinese
Abstract: Comparison studies of recombinant prourokinase(pro-UK) in various host cells,and expression vectors were carried out. Expression levels of vectors constructed in this study in different cell lines were compared. Mammalian cells expressing pro-UK were established. The levels of pro-UK expression in recombinant Namalwa, Vero and Sp2/0 cells are 200,12.5 and 50 IU/( 106 cells 24h). pro-UK purities separated by immunoaffinity chromatograph are above 90 %. Immunoabsorbent assay showed the ratio of pro-UK in CHO cells is lower than that from Vero and Namalwa cells. This study provide new host cells for pro-UK production.