Functional and Structural Study of the Prokaryotic Enhancer-like Element VV16 from Vaccinia Virus Genome
10.3321/j.issn:1000-3061.2000.03.010
- VernacularTitle:痘苗病毒VV16原核增强子样序列的克隆及其结构与功能的研究
- Author:
Xiao-Lin BI
1
;
Ru CAO
;
Jian-Xin HE
;
Feng HAN
;
Shu-Hua WU
Author Information
1. Chinese Academy of Preventive Medicine
- Keywords:
Vaccinia virus;
prokaryotic enhancer-liker element;
deletion mutagenesis;
E. coli
- From:
Chinese Journal of Biotechnology
2000;16(3):337-340
- CountryChina
- Language:Chinese
-
Abstract:
An enhancer-like element VV16 from Vaccinia virus genome DNA was obtained by using the plasmid with CAT reporter gene. Sequence analysis showed the element of 112bp is a part of the DNA-dependent RNA polymerase, polyA polymerase and DNA polymerase (RPO30 gene). It contains 4 AT-rich regions. Detection of β-galactosidase activity showed that VV16 in the positive direction can increase the activity 9.0 times and VV16 in the negative direction can increase 4.1 times. The RNA dot blotting confirmed the enhancing activity of the element are on the transcription level. DNA deletion experiment indicated the sequences of 10bp at the 5′ end and 12bp at the 3′ end in the element are important to its function and the sequence from nt76 to nt82 is essential to its activity.
